Acta Scientiae Veterinariae 怎么样-刊鹿论文编译

Detection and Characterization of Bovine Rumen Microorganisms Resistant to Sodium Fluoroacetate

Abstract:

Background : Poisoning of animals due to toxic plants is found in Brazil and other countries. One of the known toxic plants in Brazil, with the active ingredient sodium fluoroacetate (SF), is Palicourea marcgravii . Dehalogenases that inactivate the fluor-carbon bonds are enzymes found in microorganisms and may prevent intoxication. This study evaluated the occurrence of rumen microorganisms naturally resistant to SF. Materials, Methods & Results : Two samples of rumen fluid of cattle from the Experimental Farm of Federal University of Mato Grosso fed with Brachiaria sp. were obtained via fistula in flasks. An aliquot of 2 mL was placed in a microtube and centrifuged at 9000 g for 1 min. Then, the sample was inoculated into 2 tubes, one containing 100 µL of clarified rumen fluid in 2 mL of modified liquid culture medium (0.1% ammonium sulfate, 0.1% potassium phosphate monobasic, 0.05% sodium phosphate dibasic, 0.01% magnesium sulfate, 0.01% yeast extract, pH 7.0) and 0.4% of SF and the other sample containing 2 mL of liquid culture medium and 100 µL of clarified rumen fluid. The 2 samples were incubated at 40°C for 24 h. Dilutions were performed under the same conditions every 24 h until the attainment of microorganisms resistant to SF, and the finaldilution containing 50 µL of each sample was plated in the middle containing SF (0.4%) and incubated at 40°C for 24 h for the isolation of bacteria. The bacterial colonies resistant to SF were identified by morphological methods, stained, and subjected to DNA extraction sequencing using the universal primers 27f and 1492r (16S rDNA) for the identification of the bacterial genus using Blast DNA identity analysis. These bacteria were cultured with and without SF (0.4%), and the presence of fluoride ions was detected by an ion-selective electrode (fluoride) during incubation for 0, 30, 60, 90, and 120 min. Two resistant microorganisms were isolated, one was a Gram-positive coccus and the other was a Gram-positive rod. DNA sequencing identified these organisms as Enterococcus faecalis (98% identity Genbank 1358689) and Bacillus sp. (89% identity Genbank 1358671). Fluoride ions were detected more at 60-min incubation time in both E. faecalis (0.0560 ppm) and Bacillus sp. (0.0488 ppm). Bioassay protection tests were performed in mice ofthe following four groups: negative control (NC) with saline administration, positive control (PC) with administration of plant containing SF, Bacillus group (BG) with administration of plant containing SF plus Bacillus sp., and coccus group (CG) with administration of SF and E. faecalis . Clinical signs were recorded, and statistical analyses were performed to confirm the differences in the groups. Bioassay protection tests showed clinical signs of intoxication in the PC group (83.3%), BG group (100%), and CG group (16.6%) but not in the NC group (0%), with a statistical difference between GC and PC groups ( P < 0.05). Discussion : Several environmental bacteria possessing dehalogenase activity have been described, such as Pseudomonas sp., Moraxella sp., and Burkholderia sp. and Pigmentiphaga kullae and Ancylobacter dichloromethanicus isolated from the rumen. No previous study has yet reported an association between dehalogenase activity and E. faecalis , and the protection assay has been observed only in the E. faecalis group. Similar results were observed in experimental intoxication in goats that had previously consumed SF, with the microorganisms identified being Pigmentiphaga kullae and Ancylobacter dichloromethanicus . E. faecalis , isolated from the bovine rumen, exhibited a dehalogenase activity, which could help control animal poisoning by plants containing SF.

Author Listing： Maria Fernanda Aranega Pimentel;Daphine Ariadne Jesus de Paula;Franklin Riet-Correa;Valéria Dutra;Luciano Nakazato

Volume： 47

Pages： None

DOI： 10.22456/1679-9216.89907

Language： English

Journal： Acta Scientiae Veterinariae

Acta Scientiae Veterinariae

ACTA SCI VET

影响因子：0.2

是否综述期刊：否

是否OA：否

是否预警：不在预警名单内

发行时间：2002

ISSN：1678-0345

发刊频率：Tri-annual

收录数据库：SCIE/Scopus收录

出版国家/地区：BRAZIL

出版社：Universidade Federal do Rio Grande do Sul

期刊介绍

ASV is concerned with papers dealing with all aspects of disease prevention, clinical and internal medicine, pathology, surgery, epidemiology, immunology, diagnostic and therapeutic procedures, in addition to fundamental research in physiology, biochemistry, immunochemistry, genetics, cell and molecular biology applied to the veterinary field and as an interface with public health.The submission of a manuscript implies that the same work has not been published and is not under consideration for publication elsewhere. The manuscripts should be first submitted online to the Editor. There are no page charges, only a submission fee.

ASV 关注的论文涉及疾病预防、临床和内科、病理学、外科、流行病学、免疫学、诊断和治疗程序的各个方面，细胞和分子生物学应用于兽医领域并作为公共卫生的接口。提交手稿意味着同一作品尚未出版，也未考虑在其他地方出版。稿件应首先在线提交给编辑。没有页面费用，只有提交费。

年发文量	121
国人发稿量	1
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自引率	0.0%
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平均审稿周期	较慢,6-12周
版面费	-
偏重研究方向	VETERINARY SCIENCES-
期刊官网	http://www.ufrgs.br/actavet/
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