Identification of tuna species Thunnus albacares and Katsuwonus pelamis in canned products by real-time PCR method

Abstract:
Tuna species are a popular food among consumers. They are mostly sold as heat-processed canned products on the market. Different quality and price of tuna species can lead the producer to the adulteration of food products. The main difficulties in developing a method for species identification in these fish is the high similarity of DNA sequences among close relative fish species. All complete mitochondrial DNA sequences of skipjack tuna (Katsuwonus pelamis) and yellowfin tuna (Thunnus albacares) were compared to all other mitochondrial DNA sequences of tuna fish deposited in the GenBank. The most variable regions within species were determined and primers and probes were designed in this region for the species-specific DNA amplification of skipjack tuna and yellowfin tuna. Moreover, to check the content of amplifiable DNA of fish (namely tuna) in the sample, primers and a probe of mitochondrial 12S rRNA gene in the region of conservative sequence were designed. Real time PCR methods were verified by investigating 51 samples of canned tuna with the declared content of tuna species from the market; the species was confirmed in all tested samples. This method was designed to be suitable for the determination of DNA sequences especially in highly heat treated products. Yellowfin tuna, skipjack tuna, food authentication, species detection Tuna is one of the most popular fish species on the food market. Most often they are offered in the form of canned products. Among the species used in cans, skipjack (Katsuwonus pelamis) and yellowfin tuna (Thunnus albacares) dominate. Furthermore, the market offers raw or frozen fillets, most often yellowfin tuna. The different quality and price of other tuna species can lead producers to confusing the species. The Council Regulation (EEC) No. 1536/92 laying down common marketing standards for preserved tuna and bonito states specific rules for the tuna marketing. The species belonging to tuna and bonito are listed in the Annex of this Regulation. Tuna includes the genus Thunnus (T. thynnus, T. albacares, T. alalunga, T. obesus and others) and the species Euthynnus (Katsuwonus) pelamis. Sarda sp., Euthynnus sp. (except Euthynnus pelamis) and Auxis sp. are grouped among bonitos. One of the Regulation’s paragraphs says that different species may not be mixed in the same container. According to DNA analysis of tuna species, several works have described different techniques based on multiplex PCR (Bottero et al. 2007; Michelini et al. 2007; Lin and Hwang 2008), real-time PCR (Lopez and Pardo 2005, Dalmasso et al. 2007, Chuang et al. 2012; Bojolly et al. 2017) and others. To differentiate individual fish species based on DNA sequences, an increasingly widespread DNA barcoding method can be used for raw, i.e. non-heat treated samples. This method is based on the sequencing of a 655 nucleotide long stretch of mitochondrial DNA encoding a portion of the cytochrome oxidase gene, and subsequent comparison of the obtained sequence with DNA Sequence Database (Handy et al. 2011). DNA is degraded during the manufacturing process of heat treated products, so a methodology that uses shorter DNA sequences for
Author Listing： Pavel Krcmar;Zora Piskata;Eliska Servusova
Volume： 88
Pages： 323-328
DOI： 10.2754/avb201988030323
Language： English
Journal： Acta Veterinaria Brno

ACTA VETERINARIA BRNO

ACTA VET BRNO

影响因子：0.6 是否综述期刊：否 是否OA：是 是否预警：不在预警名单内 发行时间：1969 ISSN：0001-7213 发刊频率：Quarterly 收录数据库：SCIE/Scopus收录 出版国家/地区：CZECH REPUBLIC 出版社：University of Veterinary and Pharmaceutical Sciences

期刊介绍

ACTA VETERINARIA BRNO is a scientific journal of the University of Veterinary and Pharmaceutical Sciences in Brno, Czech Republic.The scientific journal Acta Veterinaria Brno is dedicated to the publication of original research findings and clinical observations in veterinary and biomedical sciences. Original scientific research articles reporting new and substantial contribution to veterinary science and original methods that have not been submitted for publication elsewhere are considered for publication. A written statement to this effect should accompany the manuscript, along with approval for publication by the author´s head of department. The authors bear full responsibility for the contents of their contribution. Book reviews are published, too.

《布尔诺兽医学报》（Acta Veterinaria Brno）是捷克共和国布尔诺兽医和制药科学大学的科学期刊，专门发表兽医和生物医学科学的原创研究成果和临床观察。报告对兽医科学有新的和实质性贡献的原始科学研究文章以及尚未在其他地方提交发表的原始方法将被考虑发表。手稿应附有一份书面声明，沿着附有作者所在部门负责人的出版批准。作者对其稿件内容承担全部责任。书评也出版了。

年发文量 53
国人发稿量 -
国人发文占比 0%
自引率 33.3%
平均录取率 容易
平均审稿周期 较慢,18-36周
版面费 -
偏重研究方向 农林科学-兽医学
期刊官网 http://actavet.vfu.cz/
投稿链接 http://actavet.vfu.cz/submissions.html

质量指标占比

研究类文章占比 OA被引用占比 撤稿占比 出版后修正文章占比
94.34% 100.00% 0.00% 0.00%

相关指数

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