ACTA VETERINARIA BRNO 期刊官网-刊鹿论文编译

Her-2 expression in feline mammary lesions

Abstract:

Due to the high occurrence of mammary neoplasms in cats, the search for prognostic factors has recently intensified. Human epidermal growth factor receptor type 2 (Her-2) is prominent among the immunohistochemical markers used for prognosis. The aim of this study was to evaluate the expression of Her-2 in mammary lesions in queens and relate the expression to the type and histological grade of mammary carcinomas. Thirty-eight cases were selected from 2006 to 2016 and were classified and histologically graded. Her-2 expression was determined through immunohistochemistry, using a semi-quantitative analysis of the slides according to the scores recommended in the Dako HercepTest. Chi-square test was used to evaluate the relationship between Her-2 expression and mammary lesion type and the type and histological grade of carcinoma (P < 0.05). The mean age was 8.5 years and young cats were affected only by benign hyperplasias and neoplasms. Undefined cats were more affected (84.37%), followed by Siamese (9.37%) and Persian (6.25%) cats. Regarding the histological classification, 18.42% of the mammary lesions were classified as hyperplasia, 18.42% as adenoma, and 63.16% as carcinoma. The most common type of carcinoma was tubulopapillary carcinoma (66.7%), followed by solid carcinoma (25%) and cribriform carcinoma (8.33%). Her-2 overexpression was observed in four samples (16.7%) of tubulopapillary carcinoma, two grade I carcinomas and two grade II carcinomas, but no relationship was observed between Her-2 expression and type of mammary lesion (P = 0.3127), carcinoma type (0.3446), or histological grade (0.6485). In conclusion, Her-2 overexpression is unrelated to histological grade and type. c-erbB-2/neu, feline, imunohistochemistry, mammary tumour Mammary neoplasms are the third most frequent tumour type in felines and most frequently affect females, adults, and uncastrated queens, with no racial predisposition (Lana et al. 2007). Several prognostic factors have already been established for these tumours, such as clinical stage, tumour size (Ito et al. 1996), histological grade (Castagnaro et al. 1998, Hughes and Dobson 2012, De Campos et al. 2015), mitosis index (Preziosi et al. 2002, Hughes and Dobson 2012), and lymphovascular invasion (Seixas et al. 2011). Moreover, in recent years, immunohistochemical markers have been used to obtain a clearer understanding of the molecular pathogenesis in feline neoplasms (Hughes and Dobson 2012). Mammary carcinomas in cats have histological features (Pérez-Alenza et al. 2004) and metastatic (Macewen 1990) and hormonal dependence (De Las Mulas et al. 2000) similar to human mammary neoplasms, indicating that results from animal models can be extrapolated to female humans (De Maria et al. 2005). Studies have indicated that human epidermal growth factor receptor type 2 (Her-2) expression in cats is associated with a larger tumour size, higher histological grade (Ordás et al. 2007), and lower survival rate (Ordás et al. 2007, De Campos et al. 2015). However, some authors have stated that, in contrast to women, Her-2 may not be involved in the carcinogenesis of feline mammary tumours and its value as a prognostic factor in queens has ACTA VET. BRNO 2019, 88: 43–47; https://doi.org/10.2754/avb201988010043 Address for correspondence: Lígia Fernandes Gundim Av. Mato Grosso, 3289 Bl 2S. Bairro Umuarama. Uberlândia, MG. Brasil. Phone: +555 34 99131 5575 E-mail: ligia_fg@hotmail.com http://actavet.vfu.cz/ been questioned because hyperplasias and malignant and benign tumours both overexpress Her-2 (Rasotto et al. 2011). Studies addressing Her-2 expression in queens are scarce and, therefore, research is necessary to elucidate its role as a prognostic factor. The aim of this study was to evaluate Her-2 immunoexpression in hyperplasias and benign and malignant neoplasms in female cats, and to relate this immunoexpression to histological type and grade, which are crucial histological indicators of mammary carcinomas. Materials and Methods A retrospective study was conducted between 2006 and 2016, examining mammary neoplasms (benign or malignant) and nonneoplastic lesions in female cats. Epidemiological data (sex, breed, animal age, and lesion size) were collected. Tumour size was grouped according to the recommendations of McNeill et al. (2009) and age was grouped according to the recommendations of Togni et al. (2013). Histological slides with mammary lesions were revised by two pathologists, classified according to the method of Misdorp et al. (1999), and histologically graded according to the method of Elston and Ellis (1993). Immunohistochemistry was performed using the streptavidinbiotin-peroxidase technique. Antigenic recovery was induced by microwave heating (ethylenediaminetetraacetic acid buffer, pH 9.0, 20 min). Subsequently, endogenous peroxidase was blocked with a 10% hydrogen peroxide solution. The first antibody (Her-2 Dako Cytomation polyclonal antibody A0485) was diluted in bovine serum albumin (BSA) at 1:400 and was incubated in a moist chamber overnight. This was followed by incubation for 30 min with a secondary antibody and with the avidin-biotin-peroxidase complex (Dako) for a further 30 min and revelation was performed with diaminobenzene (Dako K3468-1) for 3 min. Mammary carcinoma was used as a positive control and in the negative control, the primary antibody was replaced with

Author Listing： Lígia Fernandes Gundim;Tais Meziara Wilson;Nicolle Pereira Soares;Igor de Paula Castro;Mariana Ribeiro de Castro;Alessandra Aparecida Medeiros-Ronchi

Volume： 88

Pages： 43-47

DOI： 10.2754/AVB201988010043

Language： English

Journal： Acta Veterinaria Brno

ACTA VETERINARIA BRNO

ACTA VET BRNO

影响因子：0.6

是否综述期刊：否

是否OA：是

是否预警：不在预警名单内

发行时间：1969

ISSN：0001-7213

发刊频率：Quarterly

收录数据库：SCIE/Scopus收录

出版国家/地区：CZECH REPUBLIC

出版社：University of Veterinary and Pharmaceutical Sciences

期刊介绍

ACTA VETERINARIA BRNO is a scientific journal of the University of Veterinary and Pharmaceutical Sciences in Brno, Czech Republic.The scientific journal Acta Veterinaria Brno is dedicated to the publication of original research findings and clinical observations in veterinary and biomedical sciences. Original scientific research articles reporting new and substantial contribution to veterinary science and original methods that have not been submitted for publication elsewhere are considered for publication. A written statement to this effect should accompany the manuscript, along with approval for publication by the author´s head of department. The authors bear full responsibility for the contents of their contribution. Book reviews are published, too.

《布尔诺兽医学报》（Acta Veterinaria Brno）是捷克共和国布尔诺兽医和制药科学大学的科学期刊，专门发表兽医和生物医学科学的原创研究成果和临床观察。报告对兽医科学有新的和实质性贡献的原始科学研究文章以及尚未在其他地方提交发表的原始方法将被考虑发表。手稿应附有一份书面声明，沿着附有作者所在部门负责人的出版批准。作者对其稿件内容承担全部责任。书评也出版了。

年发文量	53
国人发稿量	-
国人发文占比	0%
自引率	33.3%
平均录取率	容易
平均审稿周期	较慢,18-36周
版面费	-
偏重研究方向	农林科学-兽医学
期刊官网	http://actavet.vfu.cz/
投稿链接	http://actavet.vfu.cz/submissions.html

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